Butanol Fermentation | BiotechStudies

ACETONE AND BUTANOL FERMENTATION

• Nature= strictly an anerobic
• Bacteria= clostridium acetobutylicum it is gram +ve anaerobic endospore forming bacteria

Corn meal medium=

•  it is used for the production of acetone and butanol fermentation.
• Corn meal= 8 to 10 %
• Corn powder convert into starch

Molasses medium=

•  it contain 6% sucrose, aluminium sulphate, calcium carbonate, superphosphate and cornsteep liquor.

Fermentation pass through 3 phages=
1). Rapid bacterial growth
2). Production of acetic and butyric acids
3). Evolution of CO2 and hydrogen.
Phase 1=

• rapid growth and production of acetic acid, butyric acids.
• Time period= evolution of H2 and co2. 
• For 13- 17 hrs for incubation .

Phage 2=

• rapid conversion of acids into acetone and butanol leading to decline in acidity of the medium and this phage is known as acid break.

Phase 3=

• merked decrease in gas evolution and production of acetone and butanol.

Corn meal medium yield butanol, acetone, and ethanol in 6:3:1 ratio,while for molasses the ratio is 6.5:3:5.

Production of Acetone and Butanol fermentation